Essay - Molecular Biology Clostridium Difficile How Important is the Experimental Question...

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Molecular Biology Clostridium difficile

How important is the experimental question being addressed to the field?

***** study of Matamorous, England, and Dupuy (2007) aimed to present two things. First, ***** authors would like to provide evidence that TcdC negatively regulates the synthesis of Clostridium ***** toxins. Second, the authors also wanted to show that TcdC destabilizes the TcdR-containing holoenzyme. This further suggests ***** TcdC has an ability ***** regulate toxin expression in its unique way. To understand the relevance of the two objectives incited, it must first be established what ***** is. TcdC gene is found at the right end ***** ***** pathogenicity locus (PaLoc) of C. difficile. It is ***** along with other genes such as TcdA, TcdB, and TcdR, genes that positively regulate the production ***** toxins A and B. It has *****en postulated that TcdC negatively ***** the gene expression of these toxin-producing ***** (Govind et al. 2006; Matamorous et al 2007). TcdC is highly expressed when C. difficile are found to grow exponentially, and expression is silenced ***** cells enter the stationary phase; TcdC expression is upped when other ***** genes are shut **********. There have also been studies that demonstrated mutations in the ***** genes among epidemic strains that produce both Toxins A and B (Chernak and Johnson 2005; Matamorous et al. 2007).

Evidence to support ***** two aforementioned problems is relevant ***** the field of molecular and clinical biology. TcdC ***** a rel*****tively new discovery. It is a membrane-associated protein with a rel*****tively unknown regulatory mechanism. The virulence of C. ***** is due to the ***** of ********** produced by TcdA, TcdB, and TcdR *****. There has also been a growing number of evidence ***** supports the presence of ***** ***** *****mong epidemic strains of *****. difficile. To prove that TcdC negatively regulates TcdA, TcdB and TcdR expression may allow a more in-depth understanding of the pathogenes***** of C. difficile. In turn, this may be a good foundation in developing medications ***** could combat the effects ***** this ***** pathogenic microbe. An ***** of the negative regulatory ***** of TcdC can help in addressing epidemic outbreaks.

Is the design of ***** experiment appropriate and efficient for the question? How else the ***** might ***** been designed ***** ***** an alternate design be *****tter or w*****se?

***** quantitative, experimental study essentially had two major parts. First, the authors tested ***** ability ***** ***** to repress TcdA *****. This was demonstrated ***** using in vivo and ***** vitro models. TcdC *****ive activity ***** compared to TcdR's augmentative effect in the expression of TcdA. Using C. perfingens to take the place ***** C. *****, β-glucuronidase ***** was used to measure promoter activity; the lack of β-glucuronidase activity in ***** absence ***** TcdR demonstrated that there ***** a consequent l*****ck of tcdA expression. This was in contrast to the *****crease in b-glucuronidase ***** with TcdR. However, in the culture with an overexpression of TcdC, no glucuronidase activity was demonstrated despite the presence of TcdR. *****s ***** to repress


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