Essay - Molecular Biology Clostridium Difficile How Important is the Experimental Question...


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Molecular Biology Clostridium difficile

How important is the experimental question being addressed to the field?

***** study of Matamorous, England, and Dupuy (2007) aimed to present two things. First, ***** authors would like to provide evidence that TcdC negatively regulates the synthesis of Clostridium difficile toxins. Second, the authors also wanted to show that ***** destabilizes ***** TcdR-containing holoenzyme. This further suggests that TcdC has an ability to regulate *****xin expression in its unique way. To understand the relevance of ***** two objectives incited, it must first be established what TcdC is. TcdC gene is found at the right end of ***** pathogenicity locus (PaLoc) of C. difficile. It is ***** along with other genes such as TcdA, TcdB, and TcdR, genes that positively regulate the production ***** ***** A ***** B. It has been postulated that TcdC negatively ***** the gene expression of these toxin-producing ***** (Govind et al. 2006; Matamorous et ***** 2007). ***** is highly expressed when C. ***** are found to grow exponentially, and expression is silenced ***** cells enter the stationary phase; TcdC expression is upped when ***** ***** genes are shut **********. There have ***** been studies that demonstrated mutations in the TcdC genes among epidemic strains ***** produce both *****xins A and ***** (Chernak and Johnson 2005; ***** et al. 2007).

Evidence to support ***** two aforementioned problems ***** relevant ***** the field of molecular and clinical biology. TcdC is a relatively new discovery. It is a membr*****ne-associated protein with a relatively unknown regulatory mechanism. The virulence of C. difficile is due to the synthes***** of toxins produced by Tcd*****, TcdB, and TcdR genes. There has also ***** a *****ing number of evidence that supports the presence of ***** ***** among epidemic strains of C. difficile. To prove that TcdC negatively regulates TcdA, TcdB and TcdR expression may allow a more in-depth underst*****ing of the pathogenesis of C. difficile. In turn, this may be a good foundation in developing medications ***** could combat ***** effects ***** this ***** pathogenic microbe. An understanding of the negative regulatory ***** of TcdC can help in addressing epidemic outbreaks.

Is the design of ***** experiment appropriate and efficient for the *****? How else the experiment might have been designed ***** ***** an alternate design be b*****ter or worse?

***** quantitative, experimental study essentially had two major parts. First, the authors tested ***** ability of TcdC to repress ***** expression. This was demonstrated by using in vivo and in vitro models. ***** repressive activity ***** compared to TcdR's augmentative effect in the expression of TcdA. Using C. perfingens to take ***** place of C. *****, β-glucuronidase ***** was used to measure promoter activity; the lack of β-glucuronidase activity in the absence of TcdR demonstrated that there ***** a consequent l*****ck of tcdA expression. This was in contrast to the increase in b-glucuronidase ***** with TcdR. However, in the culture with an overexpression of TcdC, no glucuronidase activity was ***** despite the ***** of TcdR. Its ***** ***** repress

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