Research Proposal: Induced Pluripotent Stem Cells to Treat

Pages: 5 (2115 words)  ·  Bibliography Sources: 10  ·  Level: Doctorate  ·  Topic: Disease  ·  Buy This Paper

¶ … Induced Pluripotent Stem Cells to Treat the Severe Neurological Disorder Angelman Syndrome

With Additional Theoretical Application to Treating Other Neurological Disorders

Concepts, Issues, and Definitions

Statement of General Topic Area: Neurological Disorders and Stem Cell Treatment

Angelman Syndrome

Induced Pluripotent Stem Cell Research

Possibilities for therapeutic cross-over benefit to other Neurological Disorders

Animal Model for Testing

Potential Therapeutic and Other Areas of Concern

Concepts, Issues, and Definitions

Statement of Purpose

The possibility of stem cell treatment as a therapeutic and/or as a cure for many disorders has been widely studied. Barriers to success have been political, social, ethical, and scientific (Fischback & Fischback, 2004). However, the field has continued to develop, with many new breakthroughs occurring within the last year, 2009. Significant progress has been made which tackles ethical and political issues, such as using induced pluripotent cells derived from somatic cells rather than embryonic cells. Induced pluripotent cells are cells that have been altered in the lab to become pluripotent, meaning they have the potential to develop into almost any type of body cell (Condic & Rao, 2008). In 2006, the first iPS cells were created using a retrovirus to modify the DNA of somatic cells. The concerns of using iPS cells revolved around the dangers associated with retroviruses. However, recent developments remove that danger by using adenoviruses instead of retroviruses to create iPS cells. (Stadtfeld, Nagaya, Utikal, Weir, & Hochedlinger, 2008). The exponential leaps in knowledge that are occurring on the scientific and medical fronts may be indicative of a soon-to-be-realized safe, effective, and widely available treatment for many diseases and disorders (Mimeault, Hauke, & Batra, 2007). The purpose of the project will be to assess whether using iPS cells to create a deliverable brain cell vehicle which expresses the gene produce known as UBE3A-E6AP, will alleviate and/or cure Angelman Syndrome.

1.2 Objectives of Research

The objectives of the research focus on the following areas:

1) To identify and assess an accurate animal model to use for iPS stem cell research in Angelman Syndrome.

2) To ascertain the proper data design to capture the relevant material.

3) To administer iPS stem cell treatment to the animal model.

4) To assess the success/failure of the research.

5) To identify areas of future study.

1.3 State of General Topic Area: Neurological Disorders and Stem Cell Treatment

Neurological disorders have been a hardship for society and individuals, with costs extending beyond healthcare costs (MacDonald, Cockerell, & Sander, 2000). Indeed, the cost of human suffering is immeasurable. It is the goal of many research scientists around the world to find answers the underlying causes of neurological disorders, and offer a scientific basis for developing therapeutics and offering cures. Stem cell treatment is one area of great promise, and has been studied as a potential treatment in many neurological conditions, such as Alzheimer's, Parkinson's Disease, Stroke patients, Cerebral Palsy, Autism, and the more rare genetic disorders, such as Angelman Syndrome (Rossi & Cattaneo, 401-409). It is with much critical thought to offer this point of question: there exists more than some slight potential of these disorders sharing common biological markers within the brain, and therefore offer the thought that what might be a viable therapeutic in one area, may indeed hold potential in neurological conditions overall (Abuhatzira, Shemer, & Razin, 2009).

1.4 The Research Problem

As Angelman Syndrome is a neurological disorder with a genetic basis, as well as being a disorder of brain-specific imprinting, the challenge of this proposal is to present sufficient evidence that using iPS-derived stem cells to grow mature, genetically normal brain cells, is viable. Moreover, administering this particular type of stem cell to an animal model of Angelman Syndrome, in order to assess the potential benefits, is a goal of this project.

2. Literature Review

2.1 Angelman Syndrome

Angelman Syndrome is a neuro-genetic disorder, affecting approximately 1 in 10,000 to 1-15,000 live births (MB, K, LK, & K., 1995). Angelman Syndrome (or AS) is a neurodevelopmental disorder characterized by global developmental delays and severe speech impairment. A few individuals with AS develop functional speech though often the mode of communication is through a mixture of gestures, eye gaze, adapted sign language and augmentative communication devices.

Individuals with AS have gross and fine motor developmental delay and severe intellectual disabilities. Current research suggests that neuronal development occurs correctly in the brain in AS, but neuronal functioning is impaired; the implication is that the 'hardware' is functionally correct with no gross physiologic abnormalities, though the actual learning and memory pathway is impacted. This neuronal impairment impacts the individual's ability to learn; skills are therefore acquired less rapidly than in age-matched peers. 'AS' individuals continue to progress throughout their lifespan in terms of learning and overall ability, yet comparisons of adults with AS vs. children with AS reveals a clinical worsening of the phenotype. This may be due to the brain developing around a dendritic defect, as well as the destructive power of intractable epilepsy (Dindot, Antalffy, Meenakshi, & Beaudet, 2008). Indeed, their ability to learn is greatly enhanced through environmental enrichment, social attachments, and repetitive learning strategies.

Individuals with AS have a movement and balance disorder which ranges from mild to severe. Walking skills typically develop after age 3, with even later onsets of walking recorded in the literature. The movement disorder can be mild or severe. Most individuals with AS have a seizure disorder. Intractable epilepsy is a primary area of concern in managing the outcome of Angelman Syndrome (Galanopoulou, 2010). Feeding and sleep disorders are common. Many individuals with AS have an unusual attraction to water, perhaps due to abnormally firing and forming neural pathways that blend sensory inputs and outputs.

2.2 Induced Pluripotent Stem Cell Research

Induced pluripotent stem cells have been manipulated in the laboratory to become pluripotent. Pluripotent stem cells have the potential to develop into virtually any other kind of body cell. The only naturally occurring pluripotent stem cells are embryonic stem cells (ES cells).

It is the hope of scientific research in this field that induced pluripotent stem cells (iPS cells) will provide a source of artificial pluripotent cells. The mechanism involved in producing these cells is through genetically coaxing an ordinary body cell, called a somatic cell, into becoming a stem cell. Somatic cells lack the ethical problems that embryonic cells have, though hypothetically they should have the same potential to treat diseases, ailments and injuries in many fields of medicine (Dobkin, 2007).

Induced pluripotent cells could theoretically be used for the identical purposes as embryonic stem cells. IPS cells have several advantages over embryonic stem cells. They can be autologous (derived from the host's own cells) and therefore would not cause an immune response. They can be isolated from patients with genetic diseases, such as Angelman Syndrome, to further research into those diseases. Chiefly, iPS cells are free of the ethical constraints that cast a pall over the use of ES cells (Condic & Rao, 2008). Moreover, they are obtained without the destruction of embryos.

2.3 Possibilities for therapeutic cross-over benefit to other Neurological Disorders

Research has indicated that there may be links underlying the cognitive deficits of various neurological disorders, such as Rett Syndrome, Angelman Syndrome, Autism, and others (Abuhatzira, Shemer, & Razin, 2009). Moreover, as the research accumulates on Alzheimer's Disease and Parkinson's Disease, we may even see common neuronal pathways involved in the etiologies of various neurological disorders and neurological diseases. A treatment in one field of study, may indeed hold great promise for treating another disorder using the same methods. Such is the reach of science and technology as scientists continue to diligently gather information on the causes of various neurological disorders.

3. Research Methodology

3.1 Animal Model for Testing

This research will utilize a scientifically-vetted mouse model of Angelman Syndrome, with unique properties. This mouse model will be null-model UBE3A mouse, which for all intents and purposes, is a deletion mouse model. This animal model does not have the maternally active copy of the UBE3A gene. Additionally, the early generation UBE3A-null models led to a demand for a model that held all the functions of the null-model, while including the ability to "report" on the status of UBE3A via a fluorescent signature marker in the brain, where UBE3A was active (Heck, Zhao, Snigdha, & LeDoux, 2008). This newer generation of the UBE3A-null model is called the UBE3A Reporter Mouse. The advantage is that, in vivo, the research scientist is able to stain the slices and view any neural genetic activity of an attempted rescue of the phenotype, in order to ascertain any success and/or to what degree success occurred (Landers, Calciano, Colosi, Wagstaff, Glatt-Deeley, & Lalande, 2005). This project is proposing to use this UBE3A Reporter Mouse Model to assess if iPS treatment rescues the motor and cognitive deficits associated with Angelman Syndrome.

3.2 Hypotheses:

H1: Transplanting iPS stem cells (from wildtype mice) into the UBE3A Reporter Mouse will result in widespread differentiation of the iPS cells into UBE3A-maternally active neurons,

H2: Following iPS… [END OF PREVIEW]

Four Different Ordering Options:

?
Which Option Should I Choose?

1.  Buy the full, 5-page paper:  $28.88

or

2.  Buy + remove from all search engines
(Google, Yahoo, Bing) for 30 days:  $38.88

or

3.  Access all 175,000+ papers:  $41.97/mo

(Already a member?  Click to download the paper!)

or

4.  Let us write a NEW paper for you!

Ask Us to Write a New Paper
Most popular!

Stem Cell Research Term Paper


Stem Cell Research -- Ethical Issues Term Paper


Stem Cell Research the Field of Regenerative Thesis


Stem Cell Research Term Paper


Stem Cell Research and the Future Thesis


View 25 other related papers  >>

Cite This Research Proposal:

APA Format

Induced Pluripotent Stem Cells to Treat.  (2010, April 2).  Retrieved June 17, 2019, from https://www.essaytown.com/subjects/paper/induced-pluripotent-stem-cells-treat/347

MLA Format

"Induced Pluripotent Stem Cells to Treat."  2 April 2010.  Web.  17 June 2019. <https://www.essaytown.com/subjects/paper/induced-pluripotent-stem-cells-treat/347>.

Chicago Format

"Induced Pluripotent Stem Cells to Treat."  Essaytown.com.  April 2, 2010.  Accessed June 17, 2019.
https://www.essaytown.com/subjects/paper/induced-pluripotent-stem-cells-treat/347.