Plasmids "Construction of the Mobilizable Article Review

Pages: 3 (853 words)  ·  Bibliography Sources: 1  ·  File: .docx  ·  Level: College Junior  ·  Topic: Genetics

SAMPLE EXCERPT . . .
"This plasmid has all the genes of its parental pMV158 and harbors the gfp gene under the control of the Pm inducible promoter." (Nieto, 2003, p. 283) In other words, this plasmid is the pMV158 plasmid but with a gfp gene under to the control of maltose inserted into it.

With the pMV158gfp plasmid created, the authors then had to test whether or not this plasmid had the ability to transfer between bacteria, or mobilize, and at what frequency, if any, this occurred. The plasmid pMV158 had previously been mobilized between pneumococcal and lactococcal strains of bacteria by means of incorporation into a separate auxiliary plasmid. The authors needed to use such an auxiliary plasmid to test whether their pMV158gfp plasmid could be transferred between three different species of Gram-positive bacteria: S. pneumoniae, L. lactis, and E. faecalis. They used pAM?1 as their auxiliary plasmid with the pMV158 and their pMV158gfp used as the replicons to be transferred. Nieto and Espinosa then used a newly developed mobilzation assay "based on the nutritional differences between pneumococcal and lactococcal" to select for transconjugants, or bacteria that have incorporated the new DNA into their cells. (Nieto 2003, p. 283) And by growing the bacteria in maltose, fluorescence could be used to test for the incorporation of the plasmids. According to the authors, all three strains of bacteria showed a measurable fluorescence "although cells of E. faecalis/pMV158GFP exhibited such a high level of fluorescence that the color was appreciable when colonies were illuminated with an UV lamp." (Nieto, 2003, p. 284)Download full Download Microsoft Word File
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Article Review on Plasmids "Construction of the Mobilizable Assignment

With the creation of pMV158GFP a new tool has been developed that can be used to observe the processes of bacteria, especially infectious bacteria. And since its development, pMV158GFP has been transferred to more that a dozen species of bacteria. It has also been used to develop a test for the "colonization and persistence of lactic acid bacteria in dairy products," using fluorescence as an indicator during the production of cheese. (Nieto, 2003, p. 285) And with a plasmid that can be mobilize between bacteria containing the fluorescence marker gfp, the future application of this tool is almost limitless when investigating the processes of bacteria.

References

Nieto, Concepcion, and Manuel Espinoza. (2003). Construction of the mobilizable plasmid pMV158GFP, a derivative of pMV158 that carries the gene encoding the green fluorescent protein. Plasmid 49: 281-285. [END OF PREVIEW] . . . READ MORE

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